Rapamycin雷帕霉素
简要描述:
Rapamycin雷帕霉素用途及描述:科研试剂,广泛应用于分子生物学,药理学等科研方面。雷帕霉素(西罗mo司)是目前世界上的强效免疫抑制剂。
产品时间:2024-03-21
Rapamycin雷帕霉素参数 Rapamycin (Sirolimus) 雷帕霉素(西罗mo司)
Rapamycin雷帕霉素定义: 一种新型大环内酯类免疫抑制药物。通过与相应免疫嗜素RMBP结合抑制细胞周期G0期和G1期,阻断G1进入S期而发挥作用,其效应为:①抑制T和B细胞增殖;②抑制IL-1、IL-2、IL-6和IFN-γ诱导的淋巴细胞增殖;③抑制IgG和供者特异性抗体(细胞毒抗体)产生;④抑制单核细胞增殖。可用于抗移植排斥反应和治疗类风湿性关节炎、红斑狼疮等自身免疫病。 溶解性:可溶解于DMSO (50mg/ml)、甲醇(50 mg/ml)、乙醇、丙酮、等有机溶剂;几乎不溶于水。 作用 本品抑制由抗原和细胞因子(白介素IL-2、IL-4和IL-15)激发的T淋巴细胞的活化和增殖,它亦抑制抗体的产生。在细胞中,西罗mo司与免疫嗜素,即FK结合蛋白-12(FKBP-12)结合,生成FKBP-12免疫抑制复合物。此复合物与哺乳动物的西罗mo司BA分子(mTOR,一种关键的调节激酶)结合并抑制其活性,从而抑制细胞周期中G1期向S期的发展。 Trusted Worldwide: more than 10,000 vials of our rapamycin have been shipped to more than 2,500 laboratories worldwide since 2002. Immunosuppressant, related to FK-506, but without calcineurin inhibitory activity even when complexed to FK-506 binding protein. Selectively blocks signaling that leads to p70 S6 kinase activation (IC50 = 50 pM). Terada, N., et al. "Failure of rapamycin to block proliferation once resting cells have entered the cell cycle despite inactivation of p70 S6 kinase." J. Biol. Chem. 268: 12062-12068 (1993). Fingar, D.C., et al. "Dissociation of pp70 ribosomal protein S6 kinase from insulin-stimulated glucose transport in 3T3-L1 adipocytes." J. Biol. Chem. 268: 3005-3008 (1993). Price, D.J., et al. "Rapamycin-induced inhibition of the 70-kilodalton S6 protein kinase." Science 257: 973-977 (1992). Chung, J., et al. "Rapamycin-FKBP specifically blocks growth-dependent activation of and signaling by the 70 kd S6 protein kinases." Cell 69: 1227-1236 (1992). Lymphokine-induced cell proliferation at the G1 phase is inhibited and apoptosis in a murine B cell line is induced by rapamycin. Rapamycin arrests the Saccharomyces cerevisiae cell cycle irreversibly in the G1 phase. Morice, W.G. ,et al. "Rapamycin-induced inhibition of p34cdc2 kinase activation is associated with G1/S-phase growth arrest in T lymphocytes." J. Biol. Chem. 268: 3734-3738 (1993). Kay, J.E., et al. "Inhibition of T and B lymphocyte proliferation by rapamycin." Immunology 72: 544-549 (1991). Heitman, J., et al. "Targets for cell cycle arrest by the immunosuppressant rapamycin in yeast." Science 253: 905-909 (1991). Rapamycin extended median and maximal lifespan of both male and female mice when fed late in life. Harrison, D.E., et al. "Rapamycin fed late in life extends lifespan in genetically heterogeneous mice." Nature 460: 392-395 (2009). Rapamycin has shown activity in slowing cellular and organismal aging. Rapamycin abolished nuclear blebbing, delayed the start of cellular senescence, and improved the degradation of progerin in Hutchinson-Gilford progeria syndrome fibroblast cells. It also reduced the formation of insoluble progerin aggregates and resulted in clearance through autophagic mechanisms in normal fibroblasts. Cao, K., et al. "Rapamycin reverses cellular phenotypes and enhances mutant protein clearance in Hutchinson-Gilford progeria syndrome cells." Sci. Transl. Med. 3: 89ra58 (2011). Due to a different mechanism of action than FK506 and other immunosuppressants, rapamycin may prove to be important in organ transplant patient therapy. Fewer side effects than the standard anti-rejection treatments have been observed. Proliferation of activated T cells, but not apoptosis, is blocked by rapamycin. The induction of apoptosis of rejection-causing T cells reduces the tendency towards transplant rejection. Schwarz, C. and Oberbauer, R. "The future role of target of rapamycin inhibitors in renal transplantation." Curr Opin Urol. 12: 109-113 (2002). Wells, A.D. et al. "Requirement for T-cell apoptosis in the induction of peripheral transplantation tolerance." Nat. Med. 5: 1303-1307 (1999). Li, Y., et al. "Blocking both signal 1 and signal 2 of T-cell activation prevents apoptosis of alloreactive T cells and induction of peripheral allograft tolerance." Nat. Med. 5: 1298-1302 (1999). We have had one lot of Selleck Chemical's rapamycin analyzed by a highly experienced and expert clinical analytical laboratory that specializes in rapamycin analyses. Using liquid chromatography - mass spectrometry, they found a purity of 96.7% (cis plus trans) for a lot of Selleck's rapamycin. In contrast, we have proven our rapamycin to be greater than 99% in purity for every lot, no exceptions. More on the subject of rapamycin purity: HPLC analysis of rapamycin is somewhat complicated. Rapamycin forms several chromatographically separable species in solution, consisting perhaps of different conformers, tautomers, hydrates and/or isomers, but they are all in equilibrium with the major form, trans-rapamycin. We have shown this by collecting the individual impurity peaks in our rapamycin product and individually re-injecting them into the HPLC. In each case, upon re-injection the collected impurity peak is reduced or absent, and the major peak is again trans-rapamycin, of ~95% purity or higher, thus confirming re-equilibration back to the major trans-isomer of rapamycin. Because we find that all significant impurities (generally, those above 0.1%) in our product, including the cis-isomer, are in equilibrium with the trans isomer in the solution used for analysis, the actual purity of our product is >99% rapamycin, in all of its equilibrium forms. In contrast, material from other suppliers typically contains impurities that do not equilibrate with trans-rapamycin, and thus are genuine contaminants. These results also indicate that it is probably not possible to obtain the trans isomer in pure form, because in solution it will quickly re-equilibrate to the mixture of cis and trans. 储存条件:-20℃,避光防潮密闭干燥。 |
订购信息:
品名 | 产地 | 货号 | 规格 | 单价 | 备注 |
Rapamycin (Sirolimus) 雷帕霉素(西罗mo司) | MKbio | MZ0201-10MG | 10MG | 248 | 现货 |
Rapamycin (Sirolimus) 雷帕霉素(西罗mo司) | MKbio | MZ0201-100MG | 100MG | 680 | 现货 |
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特级胎牛血清(北美血源) | GIBCO | 16000-044 | 500ml |
特级胎牛血清(澳洲血源) | GIBCO | 10099-141 | 500ml |
普通型胎牛血清(南美血源) | Hyclone | SV30087.02 | 500ml |
特级胎牛血清(澳洲血源) | Hyclone | SH30084.03 | 500ml |
标准型胎牛血清(澳大利亚来源) | Hyclone | SH30370.03 | 500ml |
特级胎牛血清(北美血源) | Hyclone | SH30070.03 | 500ml |
胎牛血清 乌拉圭 | GIBCO | C2027050 | 500ml |
MSC QUALIFIED FBS | GIBCO | 12662029 | 500ml |
FBS, HI | GIBCO | 10438026 | 500ml |
FBS, ES CELL QUAL. USDA APPD. | GIBCO | 10439024 | 500ml |
FBS, DIALYZED, USA ORIGIN | GIBCO | 26400044 | 500ml |
FBS QUALIFIED, USA ORIGIN | GIBCO | 26140079 | 500ml |
FBS, ES CELL QUAL, USA ORIGIN | GIBCO | 16141079 | 500ml |
FBS, HI | GIBCO | 16140071 | 500ml |
巴西胎牛 | GIBCO | 12657029 | 500ml |
新生牛血清 | GIBCO | 16010159 | 500ml |
FOETAL BOVINE SERUM | Hyclone | SH30406.02 | 500ml |
New Zealand Calf Serum | Hyclone | SH30401.01 | 500ml |
FetalClone®II | Hyclone | SH30066.02 | 100ml |
STANDARD FETAL BOVINE SERUM | Hyclone | SH30088.03 | 500ml |
Newborn Bovine Calf Serum, U.S. Origin | Hyclone | SH30118.02 | 100ml |
FETAL BOVINE SERUM | Hyclone | SH30070.02 | 100ml |
COSMIC CALF®SERUM | Hyclone | SH30087.02 | 100ml |
FETALBOVINESERUM CHARACTERIZSED | Hyclone | SH30070.03E | 500ml |
FetalClone®I | Hyclone | SH30080.03 | 500ml |
FETAL BOVINE SERUM CHARACTERIZED | Hyclone | SH30396.03 | 500ml |
FETAL BOVINE SERUM | Hyclone | SH30071.03 | 500ml |
FETAL BOVINE SERUM | Hyclone | SH30071.02 | 100ml |
FetalClone®I | Hyclone | SH30080.02 | 100ml |
FETAL BOVINE SERUM CHARACTERIZSED | Hyclone | SH30070.02E | 100ml |
FETAL BOVINE SERUM | Hyclone | SH30079.02 | 100ml |
CHARCOAL/DEXTRAN TREATED FBS | Hyclone | SH30068.02 | 100ml |
CHARCOAL/DEXTRAN TREATED FBS | Hyclone | SH30068.03 | 500ml |
FetalClone®II | Hyclone | SH30066.03 | 500ml |
HyClone FetalClone®III | Hyclone | SH30109.03 | 500ml |
STANDARD FETAL BOVINE SERUM | Hyclone | SH30088.03 | 500ml |
STANDARD FETAL BOVINE SERUM | Hyclone | SH30088.02 | 100ml |
FETAL BOVINE SERUM | Hyclone | SH30079.03 | 500ml |
马血清 | Hyclone | SH30074.03 | 500ml |
1640培养基 1640 Medium | GIBCO | 31800-022 | 10X1L |
DMEM(高糖)含丙酮酸钠 | GIBCO | 12800-017 | 10X1L |
DMEM(高糖) DMEM(High Glucose) | GIBCO | 12100-046 | 10X1L |
DMEM(低糖)DMEM(Low Glucose) | GIBCO | 31600-034 | 10X1L |
MEM 培养基 | GIBCO | 41500-034 | 10X1L |
M199培养基 M199 Medium | GIBCO | 31100-035 | 10X1L |
F12培养基 | GIBCO | 21700-075 | 10X1L |
DMEM/F12培养基 | GIBCO | 12400-024 | 10X1L |
IMDM培养基 | GIBCO | 12200-036 | 10X1L |
DMEM(高糖)培养液 | Hyclone | SH30022.01B | 500ml |
DMEM(低糖) 培养液 | Hyclone | SH30021.01B | 500ml |
1640培养液 | Hyclone | SH30809.01B | 500ml |
DMEM/F12培养液 | Hyclone | SH30023.01B | 500ml |
α-MEM培养液 | Hyclone | SH30265.01B | 500ml |
MEM/EBSS培养液 | Hyclone | SH30265.01B | 500ml |
胰蛋白mei/EDTA消化液 | Hyclone | SV30042.01 | 100ml |
青/链mei素浓缩液 | Hyclone | SV30010 | 100ml |
Lipofectamine® 2000 Transfection Reagent | GIBCO | 11668-027 | 0.75ml |
Lipofectamine® 2000 Transfection Reagent | GIBCO | 11668-019 | 1.5ml |
B-27® Serum-Free Supplement (50X), Liquid | GIBCO | 17504-044 | 10ml |
N-2 Supplement (100X), Liquid | GIBCO | 17502-048 | 5ml |
G-5 Supplement (100X), Liquid | GIBCO | 17503-012 | 1ml |
SUPERSCRIPT II | invitrogen | 18064014 |
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SUPERSCRIPT III | invitrogen | 18080044 |
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SUPERSCRIPT III 1ST | invitrogen | 18080051 |
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Wizard® SV Gel and PCR Clean-Up System | promega | A9281 | 50 preps |
Wizard® SV Gel and PCR Clean-Up System | promega | A9282 | 250 preps |
Wizard® SV Gel and PCR Clean-Up System | promega | A9285 | 1000 preps |
Dual-Glo™ Luciferase Assay System | promega | E2920 | 10ml |
Dual-Glo™ Luciferase Assay System | promega | E2940 | 100ml |
Dual-Glo™ Luciferase Assay System | promega | E2980 | 10 × 100ml |
Dual-Luciferase® Reporter Assay System | promega | E1910 | 100 assays |
Dual-Luciferase® Reporter Assay System 10-Pack | promega | E1960 | 1,000 assays |
Dual-Luciferase® Reporter 1000 Assay System | promega | E1980 | 1,000 assays |
ImProm-II™ Reverse Transcriptase | promega | A3803 | 500 reactions |
ImProm-II™ Reverse Transcription System | promega | A3800 | 100 reactions |
Reverse Transcription System | promega | A3500 | 100 reactions |
CellTiter 96® Non-Radioactive Cell Proliferation Assay | promega | G4000 | 1,000 assays |
CellTiter 96® Non-Radioactive Cell Proliferation Assay | promega | G4100 | 5,000 assays |
CellTiter 96® AQueous One Solution Cell Proliferation Assay | promega | G3580 | 1,000 assays |
CellTiter 96® AQueous One Solution Cell Proliferation Assay | promega | G3581 | 5,000 assays |
CellTiter 96® AQueous One Solution Cell Proliferation Assay | promega | G3582 | 200 assays |
CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay | promega | G5421 | 1,000 assays |
CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay | promega | G5430 | 5,000 assays |
CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay | promega | G5440 | 50,000 assays |
pGEM®-T Easy Vector System I | promega | A1360 | 20 reactions |
pGEM®-T Easy Vector System II | promega | A1380 | 20 reactions |
DeadEnd™ Fluorometric TUNEL System | promega | G3250 | 60 reactions |
DeadEnd™ Colorimetric TUNEL System | promega | G7130 | 40 reactions |
DeadEnd™ Colorimetric TUNEL System | promega | G7360 | 20 reactions |
M-MLV Reverse Transcriptase | promega | M1701 | 10,000u |
M-MLV Reverse Transcriptase | promega | M1705 | 50,000u |
GoTaq® qPCR Master Mix, | promega | A6001 | 200 Reactions |
GoTaq® qPCR Master Mix, | promega | A6002 | 1000 Reactions |
Wizard® DNA Clean-Up System | promega | A7280 | 100 preps |
SV Total RNA Isolation System | promega | Z3100 | 50 preps |
SV Total RNA Isolation System | promega | Z3101 | 10 preps |
SV Total RNA Isolation System | promega | Z3105 | 250 preps |
CellTiter-Glo® Luminescent Cell Viability Assay | promega | G7570 | 10ml |
CellTiter-Glo® Luminescent Cell Viability Assay | promega | G7571 | 10 × 10ml |
CellTiter-Glo® Luminescent Cell Viability Assay | promega | G7572 | 100ml |
CellTiter-Glo® Luminescent Cell Viability Assay | promega | G7573 | 10 × 100ml |
PepTag® Non-Radioactive PKC Assay | promega | V5330 | 120 reactions |
PepTag® Non-Radioactive cAMP-Dependent Protein Kinase Assay | promega | V5340 | 120 reactions |